The University of Pittsburgh, Department of Neurology, would like to hire a Laboratory Research Technician I to join a translation cerebrovascular research lab. The laboratory Technician will perform a variety of independent laboratory work including executing experimental protocols, collecting and analyzing data, generating figures, and scientific writing. To perform these takes well, meticulous record keeping of research data and excellent interpersonal and communication skills are prerequisites. Following training from the principal investigator and/or senior research staff, the researcher is expected to perform tasks with minor supervision from the principal supervisor. The qualified candidate should have at least a bachelor's degree in biological science or equivalent. Candidates with demonstrated expertise in isolating Extracelluar Vesicles (EV's), familiarization with Nanoparticle Tracking Analysis and proteomics will be preferred.

Performs laboratory testing and some analysis on multiple types of specimen samples. Operates and properly sterilizes equipment and maintains inventory and assists with ordering.

Essential Functions:
1. Assist with experimental procedures, data collection, and analysis (80% effort):
• Perform a variety of experimental procedures independently after training. Procedures may include plasma isolation, concentration, and analysis of extracellular vesicles (EVs). Additional techniques include molecular biology and biochemistry (western blotting, immunoprecipitation, & proteomics) and cell biology (cell/tissue culture, immunofluorescence staining, & confocal microscopy).
• Analysis of research data and generation of graphs using tools such as Graphpad Prism, SAS, and SPSS.
• Generate scientific figures using BioRender and Adobe Illustrator.
• Maintain a detailed lab notebook and records of experimental procedures.
• Maintain a detailed archive of digital research records using laboratory server.

2. Administrative and other duties (20% effort):
• Maintain and order supplies as needed for research projects
• Maintain the office and laboratory area in a clean and safe condition
• Train other lab personnel in proper experiment procedures and use of equipment
• Assist in IACUC, IBC, and IRB protocol development, compliance, and approvals
• Perform other routine duties as assigned or as position requires
• Writes scientific papers and technical manuscripts for submission to scientific journals on a regular basis. Assist in developing and submitting nationally competitive research grants.

Must be able to get around a university setting.